Photophysics of a fluorescent non-natural amino acid: p-Cyanophenylalanine
نویسندگان
چکیده
منابع مشابه
Selenomethionine, p-cyanophenylalanine pairs provide a convenient, sensitive, non-perturbing fluorescent probe of local helical structure.
The use of selenomethionine (MSe)-p-cyanophenylalanine (FCN) pairs to probe protein structure is demonstrated. MSe quenches FCN fluorescence via electron transfer. Both residues can be incorporated recombinantly or by peptide synthesis. Time-resolved and steady-state fluorescence measurements demonstrate that MSe-FCN pairs provide specific local probes of helical structure.
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ژورنال
عنوان ژورنال: Chemical Physics Letters
سال: 2010
ISSN: 0009-2614
DOI: 10.1016/j.cplett.2010.01.058